Lycoming College Newman Lab AAIr Calculator

This tool calculates the Average Amino Acid Identity of bidirectional best hit proteins (AAIr) between a reference genome and up to ten comparison genomes using the output from the sequence-based comparison tool at the Rapid Annotation with Subsystem Technology (RAST) website

INSTRUCTIONS:
1)      "Export file" from RAST Sequence-Based Comparison Tool output.
2)      Click "Browse" below to select the *.tsv file.
3)      Click the "Submit" button.
4)      Copy and Paste Results Table to a Separate Spreadsheet or Word Processor Document.

A Perl script for this analysis that takes the filename on the command line and produces the results above (tab-delimited on standard output) is available here.

This file is a set of sample data to test the AAIr calculator.

When using this website or the Perl script for your study, please cite the following article:
Krebs, J.E., Gale, A.N., Sontag, T.C., Keyser, V.K., Peluso, E.M., and Newman, J.D. (2013). A Web-based Method to Calculate Average Amino Acid Identity (AAI) Between Prokaryotic Genomes. BioTechniques submitted August, 2013.