Bio 444-Biochemistry
Syllabus
| Lecture Topics | Lab Activities | |
| Week 1 Ch. 1,2 |
M-Introduction, Concept Reviews (JN & CM) F-Amino Acids, Peptide Bonds (CM) |
Breaking Down a Cell-What is in there and how is it purified and studied? |
| Week 2 Ch. 2,6 |
M-Protein Structure (JN) F-Protein Sequences & Evolution (JN) |
Protein structure and sequence analysis with Chime/Protein Explorer. |
| Week 3 Ch. 7,8 |
M-Quiz 1 M-Hemoglobin (JN) F-Enzyme Kinetics (JN) |
Selective hydrolysis with pig liver esterase |
| Week 4 Ch. 9,10 |
M-Enzyme Catalysis (CM) F-Enzyme Regulation (JN) |
Selective hydrolysis with pig liver esterase/kinetics workshop |
| Week 5 Ch. 12 |
M-Catch-up, review for exam F-Lipids(CM) |
Exam 1 Esterase lab report due |
| Week 6 Ch. 12,13 |
M-Membranes (JN) F-Membrane Transport (JN) |
Fatty acid Methyl ester (FAME) extraction and gas chromatography |
| Week 7 Ch. 14 |
M-Signal transduction (JN) F-Signal Transduction (JN) |
Quiz 2 FAME Data analysis |
| Week 8 Ch. 11,15 |
M-Carbohydrates (CM) F-Metabolic concepts |
Exam 2 FAME lab report due |
| Week 9 Ch. 16 |
M-Glycolysis (CM) F-Gluconeogenesis (CM) |
1. Induce expression, extract protein for his tag purification 2. Metabolic reconstruction from genome sequences |
| Week 10 Ch. 20,21 |
Quiz 3 M-Glycogen metabolism (JN) F-Good Friday |
1. Purify his tag protein, quantify 2. Metabolic reconstruction from genome sequences |
| Week 11 Ch. 17 |
M-Calvin cycle & pentose phosphate pathway (JN) F-Citric acid cycle (JN) |
Exam 3 |
| Week 12 Ch. 17,18 |
M-Electron transport (JN) F-Lipid catabolism (CM) |
1. SDS-PAGE of his tag protein, Beta Galactosidase |
| Week 13 Ch. 22 |
M-Lipid synthesis (CM) F-Amino acid metabolism (JN) |
Analysis of lipophilic pigments and quinones by HPLC |
| Week 14 Ch. 23,24 |
M-Integration of metabolism (JN) F-Catch up, review |
Analysis of lipophilic pigments and quinones by HPLC Metabolic reconstruction due |