Newman Lab AAI & BBH Calculator (for single .tsv files)
INSTRUCTIONS:
1) "Export file" from RAST, Seed Viewer Sequence-Based Comparison Tool (SVSBCT) output, save to your local computer.
2) Click Browse below and select the file.
3) Click the "Submit" button.
4) Copy and Paste Results Table to a Separate Spreadsheet or Word Processor Document. It is also helpful to copy the list of organisms from thr SVSBCT page
A Perl script for this analysis that takes the filename on the command line and produces
the results above (tab-delimited on standard output) is available here.
A calculator to construct (matrices of AAI and BBH values) from a set of up to 11 files using the same genomes but with each as a reference is available here.
When using this website or the Perl script for your study, please cite the following article:
Newman J.D, Krebs, J.E., Gale, A.N., Sontag, T.C., and Peluso, E.M. (2019). Simple Tools to Calculate Average Amino Acid Identity (AAI), Percentage of Orthologous Genes and Construct Venn Diagrams. Manuscript in Preparation.