Newman Lab AAI & BBH Matrix Calculator

INSTRUCTIONS:
1)      "Export file" from RAST Seed Viewer Sequence Based Comparison Tool (SVSBCT) output for up to and including 11 files.
2)      Browse for the files below. Holding the CTRL key down will allow you to select all the files at once by clicking on each of them; or click on the first file, then hold down the SHIFT key and click on the last file to select the range.
3)      Hit the "Submit" button.
4)      Copy and Paste Results Table to a Separate Spreadsheet or Word Processor Document.

Once the files have loaded, the Submit button will appear.

Sample .tsv files(must be unzipped)

A calculator to determine the Average Amino Acid Identity (AAI) and percent of the genome made up of Bidirectional Best Hits (%BBH) from a single .tsv file is available here.
When using this website or the Perl script for your study, please cite the following article:
Newman J.D, Krebs, J.E., Gale, A.N., Sontag, T.C., and Peluso, E.M. (2019). Simple Tools to Calculate Average Amino Acid Identity (AAI), Percentage of Orthologous Genes and Construct Venn Diagrams. Manuscript in Preparation.